Inhibition of endoplasmic reticulum-resident glucosidases impairs severe acute respiratory syndrome coronavirus and human coronavirus NL63 spike protein-mediated entry by altering the glycan processing of angiotensin I-converting enzyme 2.
Identifieur interne : 001298 ( Main/Exploration ); précédent : 001297; suivant : 001299Inhibition of endoplasmic reticulum-resident glucosidases impairs severe acute respiratory syndrome coronavirus and human coronavirus NL63 spike protein-mediated entry by altering the glycan processing of angiotensin I-converting enzyme 2.
Auteurs : Xuesen Zhao [États-Unis] ; Fang Guo [États-Unis] ; Mary Ann Comunale [États-Unis] ; Anand Mehta [États-Unis] ; Mohit Sehgal [États-Unis] ; Pooja Jain [États-Unis] ; Andrea Cuconati [États-Unis] ; Hanxin Lin [Canada] ; Timothy M. Block [États-Unis] ; Jinhong Chang [États-Unis] ; Ju-Tao Guo [États-Unis]Source :
- Antimicrobial agents and chemotherapy [ 1098-6596 ] ; 2015.
Descripteurs français
- KwdFr :
- Antienzymes (), Antienzymes (métabolisme), Antienzymes (pharmacologie), Antiviraux (), Antiviraux (pharmacologie), Coronavirus humain NL63 (), Coronavirus humain NL63 (pathogénicité), Glucosidases (antagonistes et inhibiteurs), Glycoprotéine de spicule des coronavirus (métabolisme), Humains, Iminosucres (), Iminosucres (pharmacologie), Interactions hôte-pathogène (), Peptidyl-Dipeptidase A (), Peptidyl-Dipeptidase A (métabolisme), Pénétration virale (), Réticulum endoplasmique (), Réticulum endoplasmique (métabolisme), Thérapie moléculaire ciblée, Virus du SRAS (), Virus du SRAS (pathogénicité).
- MESH :
- antagonistes et inhibiteurs : Glucosidases.
- métabolisme : Antienzymes, Glycoprotéine de spicule des coronavirus, Peptidyl-Dipeptidase A, Réticulum endoplasmique.
- pathogénicité : Coronavirus humain NL63, Virus du SRAS.
- pharmacologie : Antienzymes, Antiviraux, Iminosucres.
- Antienzymes, Antiviraux, Coronavirus humain NL63, Humains, Iminosucres, Interactions hôte-pathogène, Peptidyl-Dipeptidase A, Pénétration virale, Réticulum endoplasmique, Thérapie moléculaire ciblée, Virus du SRAS.
English descriptors
- KwdEn :
- Antiviral Agents (chemistry), Antiviral Agents (pharmacology), Coronavirus NL63, Human (drug effects), Coronavirus NL63, Human (pathogenicity), Endoplasmic Reticulum (drug effects), Endoplasmic Reticulum (metabolism), Enzyme Inhibitors (chemistry), Enzyme Inhibitors (metabolism), Enzyme Inhibitors (pharmacology), Glucosidases (antagonists & inhibitors), Host-Pathogen Interactions (drug effects), Humans, Imino Sugars (chemistry), Imino Sugars (pharmacology), Molecular Targeted Therapy, Peptidyl-Dipeptidase A (chemistry), Peptidyl-Dipeptidase A (metabolism), SARS Virus (drug effects), SARS Virus (pathogenicity), Spike Glycoprotein, Coronavirus (metabolism), Virus Internalization (drug effects).
- MESH :
- chemical , antagonists & inhibitors : Glucosidases.
- chemical , chemistry : Antiviral Agents, Enzyme Inhibitors, Imino Sugars, Peptidyl-Dipeptidase A.
- chemical , metabolism : Enzyme Inhibitors, Peptidyl-Dipeptidase A, Spike Glycoprotein, Coronavirus.
- chemical , pharmacology : Antiviral Agents, Enzyme Inhibitors, Imino Sugars.
- drug effects : Coronavirus NL63, Human, Endoplasmic Reticulum, Host-Pathogen Interactions, SARS Virus, Virus Internalization.
- metabolism : Endoplasmic Reticulum.
- pathogenicity : Coronavirus NL63, Human, SARS Virus.
- Humans, Molecular Targeted Therapy.
Abstract
Endoplasmic reticulum (ER)-resident glucosidases I and II sequentially trim the three terminal glucose moieties on the N-linked glycans attached to nascent glycoproteins. These reactions are the first steps of N-linked glycan processing and are essential for proper folding and function of many glycoproteins. Because most of the viral envelope glycoproteins contain N-linked glycans, inhibition of ER glucosidases with derivatives of 1-deoxynojirimycin, i.e., iminosugars, efficiently disrupts the morphogenesis of a broad spectrum of enveloped viruses. However, like viral envelope proteins, the cellular receptors of many viruses are also glycoproteins. It is therefore possible that inhibition of ER glucosidases not only compromises virion production but also disrupts expression and function of viral receptors and thus inhibits virus entry into host cells. Indeed, we demonstrate here that iminosugar treatment altered the N-linked glycan structure of angiotensin I-converting enzyme 2 (ACE2), which did not affect its expression on the cell surface or its binding of the severe acute respiratory syndrome coronavirus (SARS-CoV) spike glycoprotein. However, alteration of N-linked glycans of ACE2 impaired its ability to support the transduction of SARS-CoV and human coronavirus NL63 (HCoV-NL63) spike glycoprotein-pseudotyped lentiviral particles by disruption of the viral envelope protein-triggered membrane fusion. Hence, in addition to reducing the production of infectious virions, inhibition of ER glucosidases also impairs the entry of selected viruses via a post-receptor-binding mechanism.
DOI: 10.1128/AAC.03999-14
PubMed: 25348530
Affiliations:
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Blumberg Institute, Hepatitis B Foundation, Doylestown, Pennsylvania, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Baruch S. 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Block</name><affiliation wicri:level="2"><nlm:affiliation>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA Baruch S. Blumberg Institute, Hepatitis B Foundation, Doylestown, Pennsylvania, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA Baruch S. Blumberg Institute, Hepatitis B Foundation, Doylestown, Pennsylvania</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Chang, Jinhong" sort="Chang, Jinhong" uniqKey="Chang J" first="Jinhong" last="Chang">Jinhong Chang</name><affiliation wicri:level="2"><nlm:affiliation>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Guo, Ju Tao" sort="Guo, Ju Tao" uniqKey="Guo J" first="Ju-Tao" last="Guo">Ju-Tao Guo</name><affiliation wicri:level="2"><nlm:affiliation>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA Ju-Tao.Guo@drexelmed.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25348530</idno><idno type="pmid">25348530</idno><idno type="doi">10.1128/AAC.03999-14</idno><idno type="wicri:Area/PubMed/Corpus">000F11</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000F11</idno><idno type="wicri:Area/PubMed/Curation">000F11</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000F11</idno><idno type="wicri:Area/PubMed/Checkpoint">000E03</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000E03</idno><idno type="wicri:Area/Ncbi/Merge">002979</idno><idno type="wicri:Area/Ncbi/Curation">002979</idno><idno type="wicri:Area/Ncbi/Checkpoint">002979</idno><idno type="wicri:Area/Main/Merge">001300</idno><idno type="wicri:Area/Main/Curation">001298</idno><idno type="wicri:Area/Main/Exploration">001298</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Inhibition of endoplasmic reticulum-resident glucosidases impairs severe acute respiratory syndrome coronavirus and human coronavirus NL63 spike protein-mediated entry by altering the glycan processing of angiotensin I-converting enzyme 2.</title><author><name sortKey="Zhao, Xuesen" sort="Zhao, Xuesen" uniqKey="Zhao X" first="Xuesen" last="Zhao">Xuesen Zhao</name><affiliation wicri:level="2"><nlm:affiliation>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA Baruch S. Blumberg Institute, Hepatitis B Foundation, Doylestown, Pennsylvania, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA Baruch S. Blumberg Institute, Hepatitis B Foundation, Doylestown, Pennsylvania</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Guo, Fang" sort="Guo, Fang" uniqKey="Guo F" first="Fang" last="Guo">Fang Guo</name><affiliation wicri:level="2"><nlm:affiliation>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Comunale, Mary Ann" sort="Comunale, Mary Ann" uniqKey="Comunale M" first="Mary Ann" last="Comunale">Mary Ann Comunale</name><affiliation wicri:level="2"><nlm:affiliation>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Mehta, Anand" sort="Mehta, Anand" uniqKey="Mehta A" first="Anand" last="Mehta">Anand Mehta</name><affiliation wicri:level="2"><nlm:affiliation>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Sehgal, Mohit" sort="Sehgal, Mohit" uniqKey="Sehgal M" first="Mohit" last="Sehgal">Mohit Sehgal</name><affiliation wicri:level="2"><nlm:affiliation>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Jain, Pooja" sort="Jain, Pooja" uniqKey="Jain P" first="Pooja" last="Jain">Pooja Jain</name><affiliation wicri:level="2"><nlm:affiliation>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Cuconati, Andrea" sort="Cuconati, Andrea" uniqKey="Cuconati A" first="Andrea" last="Cuconati">Andrea Cuconati</name><affiliation wicri:level="2"><nlm:affiliation>Baruch S. Blumberg Institute, Hepatitis B Foundation, Doylestown, Pennsylvania, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Baruch S. Blumberg Institute, Hepatitis B Foundation, Doylestown, Pennsylvania</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Lin, Hanxin" sort="Lin, Hanxin" uniqKey="Lin H" first="Hanxin" last="Lin">Hanxin Lin</name><affiliation wicri:level="4"><nlm:affiliation>Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Pathology and Molecular Medicine, McMaster University, Hamilton</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Block, Timothy M" sort="Block, Timothy M" uniqKey="Block T" first="Timothy M" last="Block">Timothy M. Block</name><affiliation wicri:level="2"><nlm:affiliation>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA Baruch S. Blumberg Institute, Hepatitis B Foundation, Doylestown, Pennsylvania, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA Baruch S. Blumberg Institute, Hepatitis B Foundation, Doylestown, Pennsylvania</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Chang, Jinhong" sort="Chang, Jinhong" uniqKey="Chang J" first="Jinhong" last="Chang">Jinhong Chang</name><affiliation wicri:level="2"><nlm:affiliation>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Guo, Ju Tao" sort="Guo, Ju Tao" uniqKey="Guo J" first="Ju-Tao" last="Guo">Ju-Tao Guo</name><affiliation wicri:level="2"><nlm:affiliation>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania, USA Ju-Tao.Guo@drexelmed.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author></analytic><series><title level="j">Antimicrobial agents and chemotherapy</title><idno type="eISSN">1098-6596</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antiviral Agents (chemistry)</term><term>Antiviral Agents (pharmacology)</term><term>Coronavirus NL63, Human (drug effects)</term><term>Coronavirus NL63, Human (pathogenicity)</term><term>Endoplasmic Reticulum (drug effects)</term><term>Endoplasmic Reticulum (metabolism)</term><term>Enzyme Inhibitors (chemistry)</term><term>Enzyme Inhibitors (metabolism)</term><term>Enzyme Inhibitors (pharmacology)</term><term>Glucosidases (antagonists & inhibitors)</term><term>Host-Pathogen Interactions (drug effects)</term><term>Humans</term><term>Imino Sugars (chemistry)</term><term>Imino Sugars (pharmacology)</term><term>Molecular Targeted Therapy</term><term>Peptidyl-Dipeptidase A (chemistry)</term><term>Peptidyl-Dipeptidase A (metabolism)</term><term>SARS Virus (drug effects)</term><term>SARS Virus (pathogenicity)</term><term>Spike Glycoprotein, Coronavirus (metabolism)</term><term>Virus Internalization (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antienzymes ()</term><term>Antienzymes (métabolisme)</term><term>Antienzymes (pharmacologie)</term><term>Antiviraux ()</term><term>Antiviraux (pharmacologie)</term><term>Coronavirus humain NL63 ()</term><term>Coronavirus humain NL63 (pathogénicité)</term><term>Glucosidases (antagonistes et inhibiteurs)</term><term>Glycoprotéine de spicule des coronavirus (métabolisme)</term><term>Humains</term><term>Iminosucres ()</term><term>Iminosucres (pharmacologie)</term><term>Interactions hôte-pathogène ()</term><term>Peptidyl-Dipeptidase A ()</term><term>Peptidyl-Dipeptidase A (métabolisme)</term><term>Pénétration virale ()</term><term>Réticulum endoplasmique ()</term><term>Réticulum endoplasmique (métabolisme)</term><term>Thérapie moléculaire ciblée</term><term>Virus du SRAS ()</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Glucosidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antiviral Agents</term><term>Enzyme Inhibitors</term><term>Imino Sugars</term><term>Peptidyl-Dipeptidase A</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Enzyme Inhibitors</term><term>Peptidyl-Dipeptidase A</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term><term>Enzyme Inhibitors</term><term>Imino Sugars</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Glucosidases</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Coronavirus NL63, Human</term><term>Endoplasmic Reticulum</term><term>Host-Pathogen Interactions</term><term>SARS Virus</term><term>Virus Internalization</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Endoplasmic Reticulum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antienzymes</term><term>Glycoprotéine de spicule des coronavirus</term><term>Peptidyl-Dipeptidase A</term><term>Réticulum endoplasmique</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Coronavirus NL63, Human</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronavirus humain NL63</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antienzymes</term><term>Antiviraux</term><term>Iminosucres</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Molecular Targeted Therapy</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Antienzymes</term><term>Antiviraux</term><term>Coronavirus humain NL63</term><term>Humains</term><term>Iminosucres</term><term>Interactions hôte-pathogène</term><term>Peptidyl-Dipeptidase A</term><term>Pénétration virale</term><term>Réticulum endoplasmique</term><term>Thérapie moléculaire ciblée</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Endoplasmic reticulum (ER)-resident glucosidases I and II sequentially trim the three terminal glucose moieties on the N-linked glycans attached to nascent glycoproteins. These reactions are the first steps of N-linked glycan processing and are essential for proper folding and function of many glycoproteins. Because most of the viral envelope glycoproteins contain N-linked glycans, inhibition of ER glucosidases with derivatives of 1-deoxynojirimycin, i.e., iminosugars, efficiently disrupts the morphogenesis of a broad spectrum of enveloped viruses. However, like viral envelope proteins, the cellular receptors of many viruses are also glycoproteins. It is therefore possible that inhibition of ER glucosidases not only compromises virion production but also disrupts expression and function of viral receptors and thus inhibits virus entry into host cells. Indeed, we demonstrate here that iminosugar treatment altered the N-linked glycan structure of angiotensin I-converting enzyme 2 (ACE2), which did not affect its expression on the cell surface or its binding of the severe acute respiratory syndrome coronavirus (SARS-CoV) spike glycoprotein. However, alteration of N-linked glycans of ACE2 impaired its ability to support the transduction of SARS-CoV and human coronavirus NL63 (HCoV-NL63) spike glycoprotein-pseudotyped lentiviral particles by disruption of the viral envelope protein-triggered membrane fusion. Hence, in addition to reducing the production of infectious virions, inhibition of ER glucosidases also impairs the entry of selected viruses via a post-receptor-binding mechanism. </div></front></TEI><affiliations><list><country><li>Canada</li><li>États-Unis</li></country><region><li>Ontario</li><li>Pennsylvanie</li></region><settlement><li>Hamilton (Ontario)</li></settlement><orgName><li>Université McMaster</li></orgName></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Zhao, Xuesen" sort="Zhao, Xuesen" uniqKey="Zhao X" first="Xuesen" last="Zhao">Xuesen Zhao</name></region><name sortKey="Block, Timothy M" sort="Block, Timothy M" uniqKey="Block T" first="Timothy M" last="Block">Timothy M. Block</name><name sortKey="Chang, Jinhong" sort="Chang, Jinhong" uniqKey="Chang J" first="Jinhong" last="Chang">Jinhong Chang</name><name sortKey="Comunale, Mary Ann" sort="Comunale, Mary Ann" uniqKey="Comunale M" first="Mary Ann" last="Comunale">Mary Ann Comunale</name><name sortKey="Cuconati, Andrea" sort="Cuconati, Andrea" uniqKey="Cuconati A" first="Andrea" last="Cuconati">Andrea Cuconati</name><name sortKey="Guo, Fang" sort="Guo, Fang" uniqKey="Guo F" first="Fang" last="Guo">Fang Guo</name><name sortKey="Guo, Ju Tao" sort="Guo, Ju Tao" uniqKey="Guo J" first="Ju-Tao" last="Guo">Ju-Tao Guo</name><name sortKey="Jain, Pooja" sort="Jain, Pooja" uniqKey="Jain P" first="Pooja" last="Jain">Pooja Jain</name><name sortKey="Mehta, Anand" sort="Mehta, Anand" uniqKey="Mehta A" first="Anand" last="Mehta">Anand Mehta</name><name sortKey="Sehgal, Mohit" sort="Sehgal, Mohit" uniqKey="Sehgal M" first="Mohit" last="Sehgal">Mohit Sehgal</name></country><country name="Canada"><region name="Ontario"><name sortKey="Lin, Hanxin" sort="Lin, Hanxin" uniqKey="Lin H" first="Hanxin" last="Lin">Hanxin Lin</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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